Figure 3.

The LRRK2 kinase inhibitor, MLi-2, has a small effect on retrograde lysosome trafficking in the distal and proximal axon. Quantification of the mean speed of anterograde and retrograde lysosomes in the distal and proximal axons of Mut1 neurons without and with 2 µM LRRK2 kinase inhibitors CZC-25146 or MLi-2 for 48 h using kymograph-based analysis (top) and object-based analysis (bottom). The green dashed line marks the median lysosomal speed of GC1 in the specific direction and position. For all graphs: Pooled individual measurements from N = at least six independent experimental replicates. Statistics were calculated using a 2-way ANOVA with the post-hoc Tukey’s test to compute the effect of compound treatment on comparison with no treatment, taking into consideration any variation between the experimental replicates. **** corresponds to p-adj ≤ 0.0001, *** to p-adj ≤ 0.001, and * to p-adj ≤ 0.05. Comparisons without a marked * were not significantly different than Mut1. The exact mean differences and p-values can be found in Table S1.