TABLE 1.

List of Bacillus sp. strains examined using the assay developed in this study

Speciesa,b	Strainb	plcR gene fragment sequenced	Avg threshold cyclee	TaqMan result (allele)
BA	89 diverse strainsc	2	26.0f	+ (T)
BC	ATCC 4342	NA	No Amp	−
BC	ATCC 14579	NA	No Amp	−
BC	D17	7	No Amp	− (G)g
BC	F3-27	NA	No Amp	−
BC	F3502/72	8	27.1	+ (G)
BC	R6	8	27.6	+ (G)
BC	ATCC 33018	10	38.5	+ (G)
BC	D5	NA	28.4	+ (G)
BC	3A	3	24.8	+ (G)
BC	S2-8	3	27.0	+ (G)
BC	F3350/87	NA	No Amp	−
BC	S2-4	NA	34.9	+ (G)
BC	R4	10	34.7	+ (G)
BC	F2-1	9	No Amp	− (G)g
BC	AH 527	6	30.0	+ (G)
BT	HD 1015	NA	No Amp	−
BT	HD 681	NA	No Amp	−
BT	HD 288	NA	No Amp	−
BT	HD 526	NA	No Amp	−
BT	97-27	4	33.0	+ (G)
BT	HD 1011	3	26.7	+ (G)
BT	HD 571	7	No Amp	− (G)g
BT	HD 682	7	No Amp	− (G)g
BT	HD 974	NA	No Amp	−
BT	HD 44	7	No Amp	− (G)g
BT	HD 30	NA	No Amp	−
BT	HD 1012	10	33.0	+ (G)
BT	HD 50	NA	No Amp	−
UNK	TET-2B	5	33.3	+ (G)

^aBA, B. anthracis; BC, B. cereus; BT, B. thuringiensis; UNK, unknown Bacillus spp.

^bSpecies and strain designations according to reference 4.

^cThe 89 diverse B. anthracis strains are described in reference 6.

^dAs represented in Fig 1. NA, strain not sequenced.

^eInput, 10 pg, average of triplicate cycle threshold values. No Amp, no amplification.

^fAverage of triplicate cycle threshold values from 10-pg input of B. anthracis Ames strain.

^gplcR genotypes (Fig. 1) were determined via sequencing using flanking primers.