TABLE 1.
PCR primers and probes used in the multiplex PCR assaya
| Primer or probe | Primer or probe sequence | Region of gene |
|---|---|---|
| Chlam-281F | 5′-GAACTGCAAGCAACACCACTG-3′ | 281-301 |
| Chlam-357R | 5′-CCATTCGGCATCTTGAAGATG-3′ | 337-357 |
| Chlam-303T | 5′-FAM-CGCTGCCGACAGATCAAATTTTGCC-BHQ1-3′ | 303-327 |
| Cat 28S rDNA 521 for | 5′-AGCAGGAGGTGTTGGAAGAG-3′ | 521-540 |
| Cat 28S rDNA 620 rev | 5′-AGGGAGAGCCTAAATCAAAGG-3′ | 600-620 |
| Cat 28S rDNA 557 probe | 5′Texas Red-TGGCTTGTGGCAGCCAAGTGT-BHQ2-3′ | 557-577 |
a
Fluorescently quenched probes were labeled with either FAM or Texas Red at the 5′ end and an appropriate black hole quencher at the 3′ end. All of the above primers and probes were synthesized by Cruachem, Ltd., Glasgow, Scotland.