FIG. 6.

Pore-forming activity of recombinant Msp in model membranes and epithelial cell membranes. (A) Single-channel conductance events due to incorporation of rMsp into black lipid bilayer model membranes. The scale shows conductance in nanosiemens on the vertical axis and time in seconds on the horizontal axis. The mean single-channel conductance at an applied voltage of 10 mV was calculated to be 10.3 nS. (B) Current recordings consistent with the gating of large conductance channels were obtained from a cell-attached patch of HeLa cell membrane with a patch electrode containing 1.5 μM rMsp. The scale associated with each recording shows the current in picoamperes on the vertical axis and time in milliseconds on the horizontal axis.