TABLE 3.

Adherence and cytotoxicity of CTLP^a to epithelial cells

Pretreatmentb	% Adherencec	SAAPNAd	% Cytotoxicitye
PBS	100 ± 9.6	100 ± 2.0	100 ± 1.6
Anti-CTLP IgG	92 ± 11.2	38 ± 3.9	87 ± 6.3
Rabbit IgG	98 ± 14.1	100 ± 0.3	97 ± 5.1
PMSF	73 ± 1.3	6.2 ± 1.5	71 ± 3.2
TLCK	100 ± 3.2	88 ± 11.6	96 ± 0.4
TPCK	85 ± 1.6	12.8 ± 3.3	83 ± 5.0
PMSF + anti-CTLP	46 ± 7.3	8.4 ± 1.0	42 ± 4.0

^aCTLP was used at 0.15 μg per ml. Assays were conducted at room temperature. 

^bSamples were pretreated by incubation for 1 h and washed by ultrafiltration. 

^cAdherence to glutaraldehyde-fixed PLE cells after a 1-h challenge, detected by anti-CTLP IgG, is expressed as a percentage of that for the PBS-treated control. 

^dChymotrypsin-like activity of pretreated CTLP before the PLE challenge, as measured by cleavage of the chromogenic substrate. 

^eCytotoxicity to PLE cells after a 1-h challenge, as measured by release of cytoplasmic LDH, is expressed as a percentage of that for the positive-lysis control.