Figure 8.

IP₃R block prevents transient effect of Ang II on CaT alternans and CaT amplitude. (A): (a) CaTs (top) and CaT ARs (bottom) recorded over time in control, in 5 µM 2-APB and during simultaneous application of 2-APB and 500 nM Ang II. (b) Mean ± SEM and individual CaT ARs recorded in control, in 2-APB and in 2-APB together with Ang II after 1 min (time point when typically the transient effect of Ang II is observed under control conditions) and after 10 min of Ang II application (N/n = 3/8, Tukey’s test). (B): Application of IP₃ receptor blocker 2-APB (5 µM) prevented the acute effect of Ang II on CaT amplitude (0.5 Hz pacing, N/n = 3/12, Tukey’s test). (C): TRPC6/3 channel blocker GSK2833503A (200 nM) had no effect on CaT amplitude and did not prevent transient decrease of CaT amplitude during ET-1 application (pacing at 0.5 Hz; N/n = 3/8, Tukey’s test).