TABLE 1.

Plasmids

Plasmid	Relevant characteristic(s)	References
pYV
pMSL41	pYV40 yscNΔ169-177 (secretion mutant)	53, 60
pPW401	pYV40 yopBΔ89-217	9
pMSL44	pYV40 yopDΔ121-165	34
pAB4052	pYV40 yopE21	34
pSI4008	pYV40 yopHΔ1-352	56
pAB406	pYV40 yopOΔ65-558	34
pAB408	pYV40 yopM23	34
pMSK41	pYV40 yopP23	34
pABL402	pYV40 yopQ17	This work
pABL403	pYV40 yopE21 yopHΔ1-352 yopOΔ65-558 yopP23 yopM23	This work
pAB409	pYV40 yopE21 yopHΔ1-352 yopOΔ65-558 yopP23 yopM23 yopBΔ89-217	This work
Clones and vectors
pMSK13	PyopE SD yopP+ (pTZ19R derivative)	34
pABL1	pBluescript KS− yopQ+ (PCR fragment encoding YopQ cloned in BamHI-HindIII sites of the vector)	This work
pABL2	pBluescript KS− yopQ17+ (directed mutagenesis of yopQ with oligo-Mipa 470)	This work
pABL3	pMRS101 yopQ17+ (SalI-XbaI of pABL2 cloned in the same sites of the vector)	This work
Suicide vectors and mutators
pKNG101	oriR6K sacBR+ oriTRK2 strAB+	30
pMRS101	oriR6K sacBR+ oriTRK2 strAB+ oriColE1 bla+	47
pAB31	pMRS101 yopHΔ1-352+	34
pAB34	pMRS101 yopOΔ65-558+	34
pAB38	pMRS101 yopM23+	34
pMSK7	pMRS101 yopP23+	34
pPW75	pKNG101 yopBΔ89-217+	51
pPW52	pKNG101 yopE21+	59
pABL4	pMRS101 yopQ17+ (NotI deletion of pABL3)	This work