Figure 2.

Olfactory neuroepithelium (ONE) processing through nasal exfoliation for biomarker analysis. ONE can be obtained through autopsies, biopsies, and, recently, by nasal exfoliation. The figure exemplifies obtaining it through nasal exfoliation, in which cells of the sample are mechanically dissociated in a selective culture medium that promotes the proliferation of olfactory neuronal precursors (ONPs) and restricts the growth of other cell populations present in the ONE. The primary culture of the ONPs can subsequently be processed for analysis by immunostaining, Western blotting, enzyme-linked immunosorbent assay, among others [180].