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Double immunodiffusion. The central well contains a rabbit hyperimmune serum against strain 25238 whole cells. Other wells: 1, LOS, 2 mg/ml; 2, dLOS-TT, 103 μg/ml; 3, dLOS-HMP, 220 μg/ml; 4 and 5, dLOS, 1 mg/ml and 200 μg/ml, respectively; 6, HMP, 500 μg/ml.
