TABLE 1.
Composition, yield, and antigenicity of conjugates
| Conjugate | Amt (μg/ml) of:
|
Molar ratio,a dLOS/protein | Yieldb (%) | A405c (hyperimmune serum) | |
|---|---|---|---|---|---|
| dLOS | Protein | ||||
| dLOS-TT | 103 | 266 | 19 | 8 | 1.9 |
| dLOS-HMP | 220 | 280 | 31 | 19 | 1.3 |
a
Expressed as moles of dLOS per mole of protein, with molecular weights of 3,000 for dLOS, 150,000 for TT, and 120,000 for HMP.
b
Based on the starting amount of dLOS and the dLOS contained in the conjugates as measured by the phenol-sulfuric acid method.
c
The antigenicity of conjugates was expressed as ELISA reactivity at A405 when the conjugates were used as coating antigens (10 μg/ml) and a rabbit immune serum was used as a binding antibody (1/8,000). LOS (10 μg/ml) showed an A405 value of 1.1 under the same conditions.