TABLE 2.
Murine antibody response to M. catarrhalis LOS elicited by conjugates
| Immunogena | Injection no. | Geometric mean (range) ELISA unitsb
|
|
|---|---|---|---|
| IgG | IgM | ||
| dLOS + TT | 1 | 1 | 1 |
| 2 | 1 | 1 (1–2) | |
| 3 | 3 | 5 (1–15) | |
| dLOS + HMP | 1 | 1 | 1 |
| 2 | 1 | 1 (1–2) | |
| 3 | 1 (1–2) | 1 | |
| dLOS-TT | 1 | 1 | 1 |
| 2 | 5 (1–19)* | 7 (2–23) | |
| 3 | 52 (6–447)** | 17 (3–91) | |
| dLOS-HMP | 1 | 1 | 1 (1–2) |
| 2 | 2 (1–8)* | 6 (2–19) | |
| 3 | 101 (15–691)** | 17 (5–63) | |
| dLOS-TT + adjuvant | 1 | 3 (1–11)* | 2 (1–4) |
| 2 | 210 (48–923)** | 16 (62–396) | |
| 3 | 470 (266–828) | 14 (8–25) | |
| dLOS-HMP + adjuvant | 1 | 3 (1–9)* | 9 (2–39) |
| 2 | 101 (26–389)** | 22 (6–84) | |
| 3 | 1,514 (299–7,658) | 32 (13–80) | |
| LOS | 1 | 1 | 3 (1–9) |
| 2 | 8 (2–40)* | 2 (1–4) | |
| 3 | 113 (20–630)** | 52 (12–230) | |
a
Ten to twenty mice for each group were given a total of three subcutaneous injections at 3-week intervals with 5 μg of conjugates, conjugates with Ribi adjuvant, LOS, or the mixture of dLOS and TT or HMP (5 μg of each). Blood samples were collected 2 weeks after the first injection and 1 week after the second and third injections.
b
The ELISA units were based on a reference serum against strain 25238; the LOS from strain 25238 was used as a coating antigen. Symbols: * versus **, P < 0.01.