TABLE 3.
Murine antibody response to proteins (TT or HMP) elicited by conjugates
| Immunogena | Injection no. | Geometric mean (range) ELISA unitsb
|
|
|---|---|---|---|
| IgG | IgM | ||
| For assay of anti-TT titer | |||
| dLOS + TT | 1 | 11 (7–18)* | 1 |
| 2 | 303 (191–481)** | 1 (1–2) | |
| 3 | 729 | 2 (1–3) | |
| dLOS-TT | 1 | 1 (1–2)* | 1 |
| 2 | 34 (21–54)** | 1 | |
| 3 | 90 (35–237) | 2 (1–3) | |
| dLOS-TT + adjuvant | 1 | 14 (6–35)* | 3 |
| 2 | 303 (191–481)** | 11 (7–18) | |
| 3 | 2,430 | 27 (13–56) | |
| For assay of anti-HMP titer | |||
| dLOS + HMP | 1 | 2 (1–6)* | 2 (1–3) |
| 2 | 377 (149–952)** | 2 (1–3) | |
| 3 | 1,403 (645–3,051) | 10 (7–14) | |
| dLOS-HMP | 1 | 1 | 1 |
| 2 | 2 (1–8)* | 1 | |
| 3 | 11 (3–43)** | 1 | |
| dLOS-HMP + adjuvant | 1 | 4 (2–9)* | 2 (1–6) |
| 2 | 52 (30–92)** | 7 (3–17) | |
| 3 | 810 (389–1,685) | 11 (7–18) | |
a
See Table 2, footnote a.
b
The ELISA units were based on a reference serum against TT or HMP; TT or HMP was used as a coating antigen. Symbols: * versus **, P < 0.01.