Figure 1.

Effect of rutin on the viability of GL15 human glioblastoma cells and C20 human microglia. The cells were treated for 24 h with different concentrations of rutin (1, 5, 10, 30, and 50 μM) or maintained under control conditions (0.05% DMSO). (A,C) Phase contrast photomicrographs of GL15 and C20 cell cultures in different treatments; scale bar = 100 μm. (B,D) Analysis by MTT test of cell viability in GL15 and C20 cells in different treatments; values were expressed as the means ± SD (n = 3); the results were compared to controls (100%), and the significance was evaluated by a one-way ANOVA test followed by the Tukey test; **** p < 0.0001.