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(A and B) Intracellular growth kinetics of L. pneumophila AA400 (Δasd), AA400c, and AA100 (wild type). ○, AA400 plus 40 μg of DAP per ml; □, AA100; ◊, AA400c; ▵, AA400, no DAP. (C and D) Comparison of intracellular survival of AA400 complemented with the PCR-generated asd (AA400cc) to that of AA100 and AA400c. CFU levels were determined by plating dilutions from each time point on BCYE agar. Some error bars cannot be seen due to their small values. □, AA100; ◊, AA400cc; ○, AA400c.
