Table 2.
Results of morphological evaluations at day 0 (D0) and day 35 (D35) in 20 feline whole blood units collected and stored for transfusion purposes
| Parameters | Time | Mean | 95% CI | SD | Median | 95% CI | Minimum | Maximum | P value for paired differences |
|---|---|---|---|---|---|---|---|---|---|
| Macrocytes (n/hpf) |
D0 | 1.3 | 0.4–2.2 | 1.9 | 0.2 | 0.1–1.8 | 0.0 | 6.9 | 0.0007 |
| D35 | 2.4 | 0.9–3.9 | 3.1 | 0.8 | 0.4–3.1 | 0.1 | 12.2 | ||
| Echinocytes (n/hpf) |
D0 | 197.5 | 141.7–253.4 | 119.2 | 183.1 | 109.3–295.0 | 10.9 | 350.0 | 0.0001 |
| D35 | 340.0 | 319.2–360.8 | 44.4 | 350.0 | 350.0–350.0 | 151.1 | 350.0 | ||
| Echinocytes (%) |
D0 | 53.6 | 38.2–69.0 | 32.9 | 63.2 | 21.3–83.3 | 6.5 | 91.0 | <0.0001 |
| D35 | 93.6 | 89.2–97.9 | 9.3 | 96.0 | 94.5–97.9 | 57.0 | 100.0 | ||
| Schistocytes (n/hpf) |
D0 | 0.5 | 0.08–1.0 | 1.0 | 0.2 | 0.1–0.4 | 0.0 | 4.6 | 0.1297 |
| D35 | 0.2 | 0.08–0.4 | 0.4 | 0.1 | 0.1–0.3 | 0.0 | 1.8 | ||
| Spherocytes (n/hpf) |
D0 | 0.1 | 0.01–0.3 | 0.3 | 0.0 | 0.0–0.1 | 0.0 | 1.3 | 0.0139 |
| D35 | 0.4 | 0.2–0.6 | 0.4 | 0.3 | 0.1–0.6 | 0.0 | 1.5 | ||
| Lysed RBCs (n/hpf) |
D0 | 2.8 | 0.6–4.9 | 4.6 | 1.3 | 0.4–2.5 | 0.1 | 17.9 | <0.0001 |
| D35 | 16.2 | 7.7–24.7 | 18.1 | 5.7 | 3.0–33.6 | 0.5 | 50.9 | ||
| RBCs with Heinz bodies (n/hpf) |
D0 | 0.2 | 0.1–0.3 | 0.2 | 0.1 | 0.1–0.3 | 0.0 | 0.9 | 0.0110 |
| D35 | 0.06 | 0.02–0.09 | 0.08 | 0.0 | 0.0–0.1 | 0.0 | 0.3 | ||
| Normal RBCs (%) | D0 | 46.3 | 30.9–61.7 | 32.9 | 36.7 | 16.6–78.6 | 9.0 | 93.5 | <0.0001 |
| D35 | 6.8 | 2.5–11.2 | 9.3 | 4.0 | 2.5–5.9 | 0.0 | 43.0 | ||
| Recognisable WBCs (%) |
D0 | 88.4 | 84.2–92.6 | 8.9 | 88.5 | 85.3–92.4 | 64.0 | 100.0 | <0.0001 |
| D35 | 9.0 | 5.1–12.9 | 8.2 | 6.0 | 3.1–11.8 | 1.0 | 31.0 |
Echinocytes were assigned a value of >350 units/high-power field (hpf) at D35 as almost all the cells showed this morphology. Statistically significant differences are shown in bold (P <0.05)
CI = confidence interval; RBCs = red blood cells; WBCs = white blood cells