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. 2024 Jan 12;13(2):140. doi: 10.3390/cells13020140

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© 2024 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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PTBP1 enhances PGK1 mRNA stability via RRM1 and RRM3 domains. (A) PTBP1 protein was pulled down via RIP assay in HGC27 cells. (B) Enrichment of PGK1 mRNA pulled down by PTBP1 protein was detected via RT-qPCR. (C,D) The actinomycin D assay showed the stability of PGK1 mRNA in AGS (C) and HGC27 cells (D) with PTBP1 knockdown at the indicated time point. (E) Schematic diagram of PTBP1 truncated mutant construction. (F) Schematic diagrams of flag-tagged PTBP1 mutants were shown. (G) The full-length or truncated PTBP1 proteins pulled down by anti-flag were verified via Western blot. (H) RIP analysis of PGK1 mRNA enrichment pulled down by flag in 293T cells transfected with full-length or truncated PTBP1 was verified via RT-qPCR. The uncropped blots are shown in File S1.