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. 2024 Jan 16;16(2):387. doi: 10.3390/cancers16020387

Figure 2.

Figure 2

Sirt6 expression is associated with gastric cancer cell death and a higher survival rate. (A) Western blot analysis showing that Sirt6 was downregulated in gastric cancer cells (SNU-638) compared with human primary stomach epithelial cells (HPSECs). (B) Quantitative analysis of cell death indicating that adenovirus-mediated overexpression of Sirt6 significantly induced cell death in gastric cancer cells (*** p < 0.001). (C) Bar graph depicting Sirt6 knockdown decreasing gastric cancer cell death (** p < 0.01). (D) Dead cells were analyzed by flow cytometry after double staining with Annexin V-FITC and PI. The amounts of apoptosis were determined as percentages of Annexin V+ and/or PI+ cells. (E) A schematic representation of the xenograft model timeline detailing the process from cell injection to treatment administration and subsequent measurement of tumor volumes. Figure created with BioRender.com. (F) Graph and (G,H) tumor tissues showing that Sirt6 overexpression led to a significant reduction in tumor volume in the gastric cancer xenograft model, with measurements taken at specified time points post treatment (**** p < 0.0001,*** p < 0.001, ** p < 0.01).