FIG. 3.

Analysis of CD59 binding to E. coli EH237 by flow cytometry. A 0.4-μg aliquot of CD59_E was incubated with 10⁹ bacteria (EH237) in the presence of 2.5 mM of Ca²⁺ at 37°C for 30 min. The washed bacteria were incubated with an antibody against CD59 (mouse MAb BRIC229) and exposed to FITC-conjugated secondary antibody (rabbit anti-mouse IgG). A total of 10,000 cells were counted, and histograms showing counts per channel (y axis) relative to fluorescence intensity (x axis) are shown. Bacteria incubated with CD59 show a mean intensity of 8.0, whereas the native bacteria show a mean intensity of 3.8 for green fluorescence.