Figure 3.

Zonulin expression in glioblastoma cell line cultures under different conditions. (a) Appearance of cultures of different cell lines in standard medium and adherent support at 96 h; (b) appearance of cultures of different cell lines in medium specific for tumorspheres and non-adherent support at 72 h; (c) zonulin expression in different cell lines under the two culture conditions. Bars represent the mean expression, and whiskers indicate the standard deviation; (d) visual depiction of Western Blot results in three glioblastoma cell lines under different culture conditions.