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. 2024 Jan 12;46(1):710–728. doi: 10.3390/cimb46010046

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© 2024 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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The newly identified inhibitors prevent DED–FADD/DED2–c-FLIP interaction (A–I). MBP-tagged FADD and His-tagged FLIP(s) were produced and purified using chromatography columns, and incubated with each inhibitor using the indicated range of concentrations, for 18 h. The different mixtures were passed on MBP-trap chromatography columns, and eluted samples were analyzed by Western Blot using antibodies directed against Histidine and MBP; C−: Negative control without FADD, C+: Positive control FADD and FLIP without any inhibitor.

The newly identified inhibitors prevent DED–FADD/DED2–c-FLIP interaction (A–I). MBP-tagged FADD and His-tagged FLIP(s) were produced and purified using chromatography columns, and incubated with each inhibitor using the indicated range of concentrations, for 18 h. The different mixtures were passed on MBP-trap chromatography columns, and eluted samples were analyzed by Western Blot using antibodies directed against Histidine and MBP; C−: Negative control without FADD, C+: Positive control FADD and FLIP without any inhibitor.