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. 2024 Jan 12;46(1):710–728. doi: 10.3390/cimb46010046

Figure 5.

Figure 5

Inhibition of c-FLIP rescues TRAIL-mediated apoptosis. A total of (A) 2 × 105 H1703-mock-transfected and H1703-FLIP (L) cells were seeded and treated with or without 100 ng/mL TRAIL for 18 h. Apoptosis was evaluated with Annexin V-PE staining by flow cytometry (n = 3). A total of (B) 2 × 105 H1703 mock-transfected and H1703-FLIP (L) cells were seeded and treated with or without 100 ng/mL TRAIL alone, inhibitors of FLIP(L) alone or with co-treatment of TRAIL/FLIP inhibitors for 18 h. Apoptosis was evaluated with Annexin V-PE staining by flow cytometry (Student’s t-test, n = 3).