TABLE 2.
Effects of washing on CDT action
| Treatmenta | %b of HeLa cells in cell cycle phase:
|
||
|---|---|---|---|
| G0/G1 | S | G2/M | |
| Not washed | 0.1 ± 0.1 | 7.4 ± 6.5 | 92.5 ± 6.5 |
| Washed at 60 min | 2.6 ± 1.2 | 7.5 ± 7.1 | 89.9 ± 8.0 |
| Washed at 40 min | 5.8 ± 1.9 | 10.0 ± 4.8 | 84.2 ± 6.4 |
| Washed at 20 min | 12.4 ± 2.7 | 14.0 ± 4.2 | 73.6 ± 6.9 |
| Medium control | 57.2 ± 5.4 | 26.8 ± 4.8 | 16.0 ± 1.8 |
a
HeLa cells were treated with either recombinant CDT or EMEM (medium control). Times reflect times after addition of CDT.
b
Values are means ± standard deviations.