Figure 5.

Biophysical stability of ECLFM1. (A) Thermal stability of ECLFM1. Phages were incubated for 1 h under different temperatures. (B) pH stability of ECLFM1. Phages were incubated for 1 h under different pH values. (C) Long-term storage stability of ECLFM1 at 4 °C and -80 °C for up to 12 months. These experiments were performed in triplicate, and data are shown as mean ± SEM. All experiments were based on the plaque-forming ability of the phage on the host lawn to assess infectious activity relative to the control under the aforementioned conditions. The results of panels (A,B) are expressed as the ratio of the tested phage titer to the original loading titer (CK). Significance was determined by one-way ANOVA with Dunnett’s multiple comparisons post-hoc test. Bars represent standard deviation (SD). Asterisks indicate significant differences (* p ≤ 0.05; **** p ≤ 0.0001).