Figure 6.

UDCA suppresses cell viability and migration in HCC cells. Relative cell cycle-related genes and BA metabolism-related gene levels using UDCA were analyzed via qPCR in SNU387 (A) and SNU182 (B). (C) Cell growth curve indicates the IC₅₀ of UDCA (left), IC₅₀ of volasertib (middle), and increasing concentrations of UDCA in a combination from 0 to 400 μM (right) in SNU387; “+vol” means add Volasertib, dashed line is IC₅₀. Images of colony formation assays in Li-7 (D) and SNU387 (E). (F) Images of the wound-healing assay in 0, 12, 24, and 48 h (left), and counts of migrated tumor cells shown on the (right), Li-7, the scale bar was same as (D). Results are means ± SD of three independent experiments. (ns, not significant, * p < 0.05, ** p < 0.01, *** p < 0.001).