FIG. 2.

SDS-PAGE and immunoblot analysis of anti-chinchilla IgA and IgG antibodies. SDS-PAGE was performed on a 12.5% polyacrylamide gel under reducing conditions (4% 2-mercaptoethanol). Lanes: 1, prestained molecular weight markers; 2, purified chinchilla IgA; 3, purified chinchilla IgG. Proteins were visualized by Rapid Coomassie blue staining (A), and immunoblotting of samples in panel A was performed with guinea pig anti-α chain of chinchilla IgA (B) or guinea pig anti-chinchilla IgG (C).