FIG. 4.

SDS-PAGE and immunoblot analysis of Ig responses in chinchilla convalescent-phase and immune sera. SDS-PAGE was performed on a 12.5% polyacrylamide gel. Lanes: 1, prestained molecular weight markers; 2, whole-cell lysate of NTHI 12; 3, HMW proteins (HMW1 + HMW2) purified from NTHI 12; 4, P6 protein purified from Hib Eagan. Proteins were visualized by Rapid Coomassie blue staining (A), and immunoblotting was performed with chinchilla convalescent-phase sera (B) or immune sera collected from animals immunized i.m. with inactivated NTHI 12 whole cells (C) or with NTHI LCDC2 (D) or normal chinchilla sera (E).