Figure 4.

The effects of H₂O₂ and/or GSLs on CSE protein expression and endogenous H₂S generation. Rat cardiac cells (H9C2) were exposed to 800 μM H₂O₂ in the presence or absence of 10 μg/mL GSL for 24 h. The cells were then collected for CSE protein expression analysis by Western blot (A), H₂S production rate analysis using lead acetate paper (B), and cysteine sulfonate production from CSE assessment using a Co-IP assay (C). In (A) * p > 0.05. In (B,C) * p < 0.05 versus control. The experiments were repeated 4 times.