Table 1.

Body parameters in NOS1 KO and NOS1/3 DKO animals compared to wild type controls.

	Wild Type	NOS1 KO	NOS1/3 DKO
body weight (g)	31.04 ± 0.57	26.13 ± 1.17 ****	26.04 ± 0.20 ****
heart weight (g)	0.153 ± 0.004	0.133 ± 0.008 *	0.121 ± 0.004 ****
heart weight/body weight (‰)	5.05 ± 0.14	5.29 ± 0.25	4.66 ± 0.18
tibial length (mm)	18.0 (17.9–18.0)	18.0 (17.0–18.1)	17.5 (17.5–18.0)
body weight/tibial length (g/mm)	1.698 ± 0.026	1.416 ± 0.058 ****	1.483 ± 0.024 ****
heart weight/tibial length (mg/mm)	8.549 ± 0.205	7.451 ± 0.362 *	6.892 ± 0.255 ***
brain weight (g)	0.456 ± 0.004	0.435 ± 0.007 *	0.430 ± 0.003 ***
brain weight/body weight (%)	1.503 ± 0.024	1.747 ± 0.082 **	1.653 ± 0.022 *
brain weight/tibial length (mg/mm)	25.48 ± 0.27	24.54 ± 0.59	24.49 ± 0.38

Mice lacking neuronal NOS (NOS1 KO) or both neuronal and endothelial NOS (NOS1/3 DKO) exhibited significantly lower body weight, heart weight, body weight/tibial length ratio, heart weight/tibial length ratio, and brain weight, whereas there was an increased brain weight/body weight ratio compared to wild type controls. The data are presented as either mean ± SE or median and interquartile range, depending on the normality of the data distribution. Statistical significance was calculated with one-way Anova test (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001). The age of animals ranged between 12 and 17 weeks. The animal numbers were n = 10, 6, and 11 for the wild type, NOS1 KO, and NOS1/3 DKO groups, respectively.