Figure 4.

Systemic noribogaine (Noribo 40; N40) treatment reduced NMDA-mediated current density in mPFC pyramidal neurons from male mice. (A,B) Graphs showing saline (black bars) and N40 (red bars) administration effect on mean EPSC paired-pulse ratio during 10 Hz stimulation (i.e., fraction of EPSC₂/EPSC₁ amplitudes) recorded from layer V mPFC pyramids from male WT (left plot) and 5HT_2A KO (right plot) mice, respectively. Individual paired-pulse ratio values are shown for each treatment as overlying (WT), rhombi (5HT_2A KO) on each bar. No significant differences were observed comparing PPR values. (C,D) Graphs showing saline (black) and N40 (red bars) administration effects on mean NMDA-mediated current density (pA/pF) values from male WT (left plot) and 5HT_2A KO (right plot) mice. Individual NMDA-mediated current density values are shown for each treatment as overlying circles (WT), rhombi (5HT_2A KO) on each bar. Note how N40 was able to significantly reduce the NMDA current density only in pyramidal neurons from WT male mice (see Table 1 for statistical comparisons). ** Mean INMDA current-density values were significantly smaller in KO compared to WT (post-hoc Tukey test; q = 6.2, p < 0.01). The values indicate mean ± SEM. The number of cells recorded for each group were included in brackets on top of each bar.