Figure 7.

Cyc1 variant connections with the Cyb respiratory subunit of Complex III. AlphaFold2-Multimer high-accuracy prediction of mouse Cyc1-Cyb of Complex III [13,14,15] with predicted alignment error (PAE) plots visualised through ChimeraX. (A) ‘cleaved Cyc1’ interaction with Cyb. (B) ‘uncleaved Cyc1’ interaction with Cyb as found in Immp2l^KD−/− KO mouse. (C) PAE plot of ‘uncleaved Cyc1’-Cyb where blue colour intensity depicts higher confidence. Upper left and lower right quadrants of PAE plot represent the confidence of the predicted structure of the unitary respiratory subunits whereas the lower left and upper right quadrants depict the confidence of the predicted respiratory subunit complex of Cyc1 (blue), Cyb (wood) and the ‘uncleaved’ signal transit peptide of Cyc1 (orange). (D) Magnified lateral view of subfigure B (above), highlighting the contact forming residues between the ‘uncleaved’ signal transit peptide (Orange) of Cyc1 (blue) and Cyb (wood). The contact forming residues are shown in green and the contacts are shown as yellow dotted lines.