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Validation of PARP1 knockdown. PARP1 was knocked down with siRNA as above. (a) Protein extracts were quantified, run on SDS PAGE and western blotted for PARP1 antibodies. (b) RNA was purified from cells and converted to cDNA, and the level of PARP1 transcript expression analyzed by qRT-PCR. *Mean of n = 3 ± SEM, p < 0.05 by T-test
