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C. difficile toxin B gene and locations of expression clones. Int. B-4 and Int. B-(1+2) each comprise a pool of two or three smaller fragments. The amino acid interval expressed in each clone is as follows: for Int. B-4, aa 10 to 330 and aa 260 to 520; for Int. B-(1+2), aa 510 to 1110, aa 820 to 1110, and aa 1110 to 1530; for Int. B-5, aa 1530 to 1750; and for Int. B-3, aa 1750 to 2360. The shaded region at the 3′ end represents the repetitive domain of the toxin B gene.
