FIG. 2.

GBSIII-specific IgG subclass antibodies in naive mice transferred with splenocytes from GBSIII-TT-immunized donors. BALB/c mice were immunized twice 3 weeks apart with GBSIII-TT conjugate vaccine; 4 weeks after the second immunization, 2 × 10⁸ spleen cells were adoptively transferred into six naive recipients. All recipient mice were immunized 24 h later with GBSIII-TT, and the IgG subclass response to the GBSIII PS was measured in serum taken 28 days after immunization. The levels of GBSIII-specific IgG subclass antibodies are reported in nanograms per milliliter.