FIG. 4.
Dose response following adoptive transfer of spleen cells from mice primed with GBSIII-TT conjugate vaccine. BALB/c mice were immunized with GBSIII-TT conjugate vaccine, and 2 × 108, 1 × 108, 2 × 107, or 2 × 106 spleen cells from these primed mice were adoptively transferred into naive recipients (groups of six mice). In the control group, 2 × 108 naive spleen cells were transferred (hatched bars). All recipient mice were immunized with GBSIII-TT, and blood was taken 0, 7, and 14 days after immunization. (A) GBSIII PS-specific IgG antibodies; (B) TT-specific IgG antibodies. The levels of GBSIII-specific and carrier TT-specific IgG antibodies after the transfer of spleen cells are reported as medians. ∗, P < 0.01 compared with recipients of splenocytes from placebo-immunized donors (Mann-Whitney test).