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. 2024 Jan 16;25(2):1101. doi: 10.3390/ijms25021101

Figure 4.

Figure 4

KMT2C and BCOR variants expression using MGB TaqMan probes. (A) Illustration and sequences of primers and TaqMan MGB probes designed to determine the expression rates of the KMT2C and BCOR genes in wild-type (WT) and mutant-type (MT) conditions. The primer sequences are identical for both WT and MT. For the probes, WT is labeled with the VIC fluorescent dye (orange circle), while MT is labeled with the FAM fluorescent dye (blue circle). To enhance specificity, fluorescently labeled probes are quenched using the MGB (yellow circle)-eclipse quencher (gray circle). These probes are denoted PrWT and PrMT. Detailed sequences are provided in the table below. The variants were measured by two different molecular techniques: (B) ddPCR and (C) real-time PCR. The data represent the means ± SD of triplicates; * indicates p < 0.05, ** indicates p < 0.01, *** indicates p < 0.001 compared to the WT 10 fg group or 0 week of each hiPSC-CM group. VIC, VIC fluorophore; FAM, FAM fluorophore; Q, nonfluorescent quencher; MGB, minor groove binding; F, forward; R, reverse; PrWT, probe for wild type; PrMT, probe for mutant type; ddPCR, digital droplet PCR; KMT2C, histone-lysine N-methyltransferase 2C; BCOR, B-cell lymphoma 6 protein corepressor; NTC, no template control; Conc, concentration; Rn, normalized reporter.