Table 2.
Inter-person precision of KMT2C and BCOR variants using ddPCR.
| Sample Type | KMT2C c.2263C > T Concentration (Copies/µL) | |||||||
|---|---|---|---|---|---|---|---|---|
| Analyst | A-1 | A-2 | A-3 | B-1 | B-2 | B-3 | Mean ± SD | %RSD |
| NTC | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 ± 0.00 | - |
| WT 10 fg | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 ± 0.00 | - |
| WT 5 fg + MT 5 fg | 93.92 | 91.73 | 94.38 | 103.07 | 99.30 | 107.51 | 98.32 ± 5.57 | 5.67 |
| MT 10fg | 210.82 | 205.19 | 199.40 | 212.75 | 217.00 | 218.40 | 210.59 ± 6.60 | 3.13 |
| Sample Type | BCOR c.1487_1500del Concentration (copies/µL) | |||||||
| Analyst | A-1 | A-2 | A-3 | B-1 | B-2 | B-3 | Mean ± SD | %RSD |
| NTC | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 ± 0.00 | - |
| WT 10 fg | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 ± 0.00 | - |
| WT 5 fg + MT 5 fg | 25.61 | 29.26 | 30.03 | 31.57 | 33.30 | 31.50 | 30.21 ± 2.42 | 8.01 |
| MT 10fg | 55.82 | 61.72 | 53.46 | 64.83 | 69.50 | 62.20 | 61.25 ± 5.36 | 8.75 |