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. 2024 Jan 16;25(2):1088. doi: 10.3390/ijms25021088

Figure 5.

Figure 5

Figure 5

TEC cytoskeleton rearrangement and mitochondrial transfer. (a) Fluorescently labeled TECs from each group, treated with adenovirus and siRNA, were imaged under a 63 × 1.4 oil microscope using laser scanning confocal microscope. G-actin (red) and F-actin (green) were visualized, and nuclei were stained with DAPI (blue). White arrows indicate adhesive spots. Scale bar = 50 μm; (b) Statistical results of F-actin and G-actin fluorescence intensity from (a); (c) Representative Western Blot images of β-ACTIN, PROFILIN-2, CYCLIND1, α-TUBLIN, VIMENTIN, and E-CADHERIN; (d) Statistical results of PROFILIN-2 levels; (e) Statistical results of E-CADHERIN levels. Protein levels were normalized using the corresponding β-ACTIN level; (f) Statistical results of mRNA levels of profilin-2. mRNA levels were normalized using GAPDH by the 2−ΔΔCt method; (g) Statistical results of mRNA levels of e-cadherin. mRNA levels were normalized using GAPDH by the 2−ΔΔCt method; (h) Representative images of TECs mitochondria (orange), thymocytes mitochondria (purple), F-actin (green), and nuclei (blue). Representative images of enlarge selected areas in the blue and yellow boxes. Scale bar = 10 μm (bigger), 5 μm (smaller). One-way ordinary ANOVA test was used in (d–g). A two-way ANOVA test was used in (b). Each symbol represents a separate data point. Data for (b,d–g) are presented as mean ± SEM from at least three independent experiments. Statistical significance was determined by comparing the NC siRNA group (* p < 0.05, ** p < 0.01), the AdCon group (^ p < 0.05, ^^ p < 0.01), and the LVCon group (## p < 0.01).