FIG. 1.

Construction of recombinant plasmid pIP67. The p67 gene of T. parva was amplified by PCR using the pUC19-based plasmid pK19p67 as a template. Both primers (see Materials and Methods) contained NsiI sites. The PCR product was then ligated into the NsiI site of pMOhly1. The resulting plasmid was designated pIP67.