Table 3.
Published feline reference intervals (RIs) for the Sysmex XT-2000iV analyser, 17 and the new adult Maine Coon-specific RIs (Sysmex XT-1800iV)
| Analyte | Published RI 17 | Outside RI (%) | Maine Coon RI |
|---|---|---|---|
| RBC-I (1012/l) | 6.7–11.2 | 65 | 4.6–8.5 |
| Haemoglobin (g/l) | 99.1–165.9 | 0 | 133.0–163.0 |
| Haematocrit (l/l) | 0.29–0.51 | 0 | 0.37–0.48 |
| MCV (fl) | 34.4–49.3 | 30 | 41.1–59.0 |
| MCH (pg/cell) | 12.3–16.4 | 0 | 12.2–15.8 |
| MCHC (g/dl) | 32.5–39.2 | 35 | 29.1–40.0 |
| WBC (109/l) | 4.0–15.2 | 0 | 5.7–15.6 |
| Neutrophils (109/l) | 1.7–8.8 | 0 | 3.4–10.8 |
| Eosinophils (109/l) | 0.1–1.2 | 0 | 0.0–0.7 |
| Lymphocytes (109/l) | 1.2–10.2 | 0 | 1.1–4.6 |
| Monocytes (109/l) | 0.1–0.6 | 10 | 0.2–1.0 |
| PLT-I (109/l) | 33–516 | 0 | 85–385 |
| Reticulocytes (%) | 0.2–1.1* | 50 | 0.2–3.3 † |
| Reticulocytes (109/l) | 19.1–107.1* | 35 | 11.3–250.0 † |
RIs were considered validated if ⩽10% (n = 0–2) of the Maine Coon values fell outside the published reference limits. RIs were rejected if >25% of the reference values (n ⩾5) fell outside the reference limits. If >10% but <25% of the reference values (n = 3–4) fell outside the reference limits, a new set of 20 animals was randomly selected and their reference values compared with the RIs. In the new set-up, if ⩽10% of the samples fell outside the RIs, then the laboratory RI was considered validated; otherwise, it was rejected. When the published RI was rejected, a new RI, reported in bold, was established following the Clinical and Laboratory Standards Institute and American Society of Veterinary Clinical Pathology guidelines15,16
Aggregate and punctate reticulocytes count, † Manual count, only aggregate reticulocytes counted
RBC-I = red blood cell count by impedance measurement; MCV= mean cell volume; MCH = mean corpuscular haemoglobin; MCHC = mean corpuscular haemoglobin concentration; WBC = white blood cells; PLT-I = platelet count by impedance measurement