Table 2.
Volume of sample required for each test and whether effusion can be used
| Test | Type of test | Antigen | Amount of sample (µl) | Performing the test | Time to result* | Antibody titre? | Can effusion be used? |
|---|---|---|---|---|---|---|---|
| FCoV Immunocomb | ELISA | FCoV | 5 | In-house | 40 mins | Yes | Yes |
| Anigen Rapid FCoV | RIM | Unknown | 10 | In-house | 10 mins | No | Not advised in instruction booklet, but does work to some extent with effusion |
| Biobest | IFAT type II FCoV | Whole virus (FCoV type II) in CRFK cells | 25 | External laboratory | >1 day | Yes | Yes |
| VDS Glasgow | IFAT type II FCoV | Whole virus (FCoV type II) in feline embryo A cells | 25 (ask for 1 ml sample but can do the test with much less) | External laboratory | >1 day | Yes | Yes |
| Speed F-Corona | RIM | Recombinant TGEV N | 40 | In-house | 15 mins | No | Yes |
| FASTest FIP | RIM | Recombinant FCoV antigens | 40 | In-house | 10 mins | No | Not advised in instruction booklet, but works well with effusions |
| Zurich | IFAT TGEV | Whole virus (TGEV Purdue) in porcine kidney cell line PD5 | 50 of plasma/serum requested; 10 used (the rest is in case the test has to be repeated) | External laboratory | >1 day | Yes | Yes |
| ENVA | IFAT TGEV | Whole virus (TGEV) in porcine kidney cells | 100 | External laboratory | >1 day | Yes | Yes |
The amount of sample each test requires is shown: the tests are shown in ascending order of quantity of sample required
VDS = Veterinary Diagnostic Services; ENVA = École Nationale Vétérinaire d’Alfort; ELISA = enzyme-linked immunosorbent assay; N = nucleocapsid antigens; RIM = rapid immunochromatographic; IFAT = immunofluorescent antibody test; TGEV = transmissible gastroenteritis virus
*
Including shipment