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. 2024 Jan 15;15(1):24. doi: 10.3390/jfb15010024

Figure 5.

Figure 5

Bacterial biofilm on TPUs. E. coli (A, a; B, a–c) and S. aureus (A, b; B, d–f) were incubated in biofilm conditions on TPU Film, Brush, and Bar Coater for 24 h at 37 °C. After removal of supernatant, viability of biofilms was determined through MTT (A). Biofilm viabilities (%) were reported with respect to the TCP control, represented by biofilm grown in medium and set as 100% (red line). Data are represented as the mean values of the replicates (n = 3) ± the standard deviation (SD), represented by the error bars. Statistical analysis (*, #) indicates the analysis vs. TCP: p < 0.001 (***), p < 0.01 (**), p < 0.05 (*). One-way analysis of variance (ANOVA), followed by Bonferroni’s test between samples (#) within bacterium, was performed: p < 0.05 (#) and p < 0.01 (##). SEM images (B) of E. coli (a–c) and S. aureus (d–f) biofilms on Film (a,d), Brush (b,e), and Bar Coater (c,f) were acquired at 6k× magnification (scale bar 8 µm), and insets at 30k× (scale bar 2 µm).