TABLE 3.
Percentage of Vδ2-positive cells among CD3 cells cocultured with different bacterial extractsa
| Extract | % of Vδ2-positive cells in two donors at an antigen concn (μg/ml) of:
|
||||
|---|---|---|---|---|---|
| 3 × 103 | 750 | 187 | 47 | 12 | |
| M. fortuitum | 24.9 | 10.4 | 3.3 | 3.4 | 5.6 |
| FSC171 | 16.1 | 7.4 | 3.3 | 5 | 5.6 |
| LVS | ND | 14.6 | 3.9 | 4.3 | 3.6 |
| None (medium) | 3.4 | ||||
a
Fresh PBL from a healthy donor were incubated with decreasing antigen concentrations. The amplification of Vγ9Vδ2 cells was estimated by fluorescence-activated cell sorter analysis with a dual-stain (CD3-phycoerythrin and Vδ2-FITC) assay after 15 days of coculturing. ND, not done (no cells were found in wells containing 3 × 103 mg of LVS per ml). All of these cocultures were used for other donors and yielded the same results.