Table 2.
Characteristics of the Five Microsatellite Loci of F. taiwana Used in This Study.
| Locus | Size Range (bps) | Motif Type 1 | Primer Sequences 2 and the Concentration of the Primers Used in the PCR Reactions | Number of Alleles | Accession Number |
|---|---|---|---|---|---|
| P1 | 138~183 | (TAA)7 | F: TCAGGCTCGTACGGTTTACT (200 nM) | 7 | MW921469 |
| R: GGAAGTCAAAATAGGAGTTTCTCAA (200 nM) | |||||
| P2 | 213~239 | (ATT)8 | F: AAATCAATAAATATTACGAGTTTTCCA (100 nM) | 8 | MW921470 |
| R: TTTTTATCGTTCAAAATCCTTCTG (200 nM) | |||||
| P3 | 256~264 | (TGA)6 | F: GGGGCCTCCTCATAAGTACC (100 nM) | 5 | MW921471 |
| R: TGCATTTCTTGCACTCCATT (200 nM) | |||||
| P4 | 348~359 | (CAA)6 + (CCA)6 | F: CCCACGCACTAACGAGAGTT (150 nM) | 5 | MW921472 |
| R: AGCGGGAGTTCCATTTCTCT (200 nM) | |||||
| P5 | 453~465 | (TTC)7 | F: TCCTCACCCCAAAAAGTGAC (100 nM) | 6 | MW921473 |
| R: AGGGAGCGACTTCAAATCAA (200 nM) |
1 The number of repeats for microsatellite markers in the PCR-amplified sequences deposited in GenBank is indicated; 2 All of the forward (F) primers have an M13 primer sequence (CACGACGTTGTAAAACGAC) extension at their 5′ ends.