TABLE 1.
Effect of coculturing SNAP with C. ruminantium-infected cells on the viability of extracellular rickettsiae
| SNAP concn (μM) | % Viability (mean ± SD) on day:
|
|||
|---|---|---|---|---|
| 1 | 2 | 4 | 7 | |
| 0 | 90 ± 8 | 82 ± 4 | 75 ± 4.5 | 82.5 ± 14 |
| 25 | 79 ± 3 | 68 ± 5 | 52 ± 4 | 57.9 ± 7 |
| 50 | 56 ± 7 | 45 ± 3 | 28 ± 2.5 | 25.5 ± 4.2 |
| 100 | 38 ± 5 | 37 ± 4 | 20 ± 3 | 5.4 ± 1 |
a
Mean viability counts of C. ruminantium EBs in the culture medium were assessed on days 1, 2, 4, and 7 postinfection in cultures incubated with different concentrations of SNAP. The viability was inversely related to the concentration of SNAP and consequently to the NO levels (r = −0.96) but was highly correlated with the number of colonies per coverslip (r = 0.97) on day 7 postinfection.