Table 4. Primer combinations used to amplify cox1 with reference, sequence, annealing temperature, and amplicon length.
| Primer | Reference | Direction | Sequence (5´-3´) | Tm (°C) | Amplicon lenght (bp) |
|---|---|---|---|---|---|
| mtd6 (C1-J-1718) | [59] | Forward | GGAGGATTTGGAAATTGATTAGTTCC | 50 | 472 |
| mtd9 (C1-N-2191) | [59] | Reverse | CCCGGTAAAATTAAAATATAAACTTC | ||
| mtd6 (C1-J-1718) | [59] | Forward | GGAGGATTTGGAAATTGATTAGTTCC | 50 | 850 |
| H7005P-R | [60] | Reverse | TGAGCTACTACRTARTATGTRTCATG | ||
| LCOP-F | [56] | Forward | AGAACWAAYCATAAAAYWATTGG | 48 | 658 |
| HCO2198 | [61] | Reverse | TAAACTTCAGGGTGACCAAAAAATCA | ||
| tRWF1 | [62] | Forward | AACTAATARCCTTCAAAG | 50 | ±860 |
| LepR1 | [63] | Reverse | TAAACTTCTGGATGTCCAAAAAATCA |
Abbreviation: Tm: primer melting temperature.