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. 2024 Jan 12;10(1):63. doi: 10.3390/jof10010063

Figure 1.

Figure 1

Phenotype and sequence confirmation of TRP1 and LIP2 disruption strains. (A) Verification of TRP1 disruption strains. Transformants that failed to grow on YNBD were trp− mutants. A representative sample is shown for the alignments of the TRP1 gene sequence from selected trp− mutants. (B) Verification of LIP2 disruption strains. Transformants that exhibited a reduced halo of triglyceride hydrolysis on YNBD-triglyceride were lip− mutans. A representative sample is shown for the alignments of the LIP2 gene sequence from selected lip− mutants. The blue represents gRNA sequence, the red represents indels, and the sequence in green rectangle represents PAM sequence.