TABLE 2.
Protection of mice by active immunization with different forms of DbpA compared with that by immunization with OspA
| Expt (mouse strain/challenge isolate) | Mouse group | Immunogena | No. of positive cultures/total no. tested
|
P39 IgGb | No. of mice infected/total no. | ||
|---|---|---|---|---|---|---|---|
| Bladder | Ear | Joint | |||||
| A (C3H/297) | 1 | His-DbpA297 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 |
| 2 | Lpp1:DbpA297 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | |
| 3 | OspAB31 | 0/5 | 0/5 | 0/5 | − | 0/5 | |
| 4 | PBS | 5/5 | 5/5 | 4/5 | + | 5/5 | |
| B (C3H/N40) | 1 | Lpp2:DbpAN40His | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 |
| 2 | OspAB31 | 5/5 | 0/5 | 0/5 | + | 5/5 | |
| 3 | E. coli BL21 (det.) | 4/4 | 4/4 | 1/4 | + | 4/4c | |
| 4 | None | 5/5 | 5/5 | 5/5 | + | 5/5 | |
| Cd (BALB/B31) | 1 | Pre-DbpA297 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 |
| 2 | OspAB31 | 0/5 | 0/5 | 0/5 | − | 0/5 | |
| 3 | E. coli JM101 (sol.) | 5/5 | 5/5 | 5/5 | ND | 5/5 | |
| 4 | None | 5/5 | 5/5 | 5/5 | + | 5/5 | |
| (C3H/B31) | 5 | Pre-DbpA297 | 5/5 | 0/5 | 0/5 | 5/5 | 5/5 |
| 6 | OspAB31 | 0/5 | 0/5 | 0/5 | − | 0/5 | |
| 7 | E. coli JM101 (sol.) | 5/5 | 5/5 | 5/5 | ND | 5/5 | |
| 8 | None | 5/5 | 5/5 | 5/5 | + | 5/5 | |
| D (C3H/B31) | 1 | Pre-DbpA297 | 5/5 | 0/5 | 0/5 | 5/5 | 5/5 |
| 2 | Lpp1:DbpA297 | 5/5 | 0/5 | 0/5 | 5/5 | 5/5 | |
| 3 | OspAB31 | 0/5 | 0/5 | 0/5 | − | 0/5 | |
| 4 | E. coli JM101 (sol.) | 5/5 | 5/5 | 5/5 | + | 5/5 | |
| 5 | E. coli BL21 (det.) | 5/5 | 5/5 | 5/5 | + | 5/5 | |
| 6 | None | 5/5 | 5/5 | 5/5 | + | 5/5 | |
| Ed (C3H/B31) | 1 | Pre-DbpA297 | 3/5 | 0/5 | 0/5 | 3/5 | 3/5 |
| 2 | His-DbpA297 | 1/5 | 0/5 | 1/5 | 1/5 | 1/5 | |
| 3 | His-DbpAB31 | 1/4 | 0/4 | 0/4 | 0/4 | 1/4c | |
| 4 | OspAB31 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | |
| 5 | E. coli M15 (sol.) | 5/5 | 5/5 | 5/5 | ND | 5/5 | |
| 6 | None | 5/5 | 5/5 | 5/5 | 5/5 | 5/5 | |
a
Immunogen doses were 20 μg for DbpA, 5 μg for OspA, and 5 μg for E. coli soluble (sol.) and detergent (det.) extracts, except 20 μg in experiment C.
b
Presence of IgG to P39 at the time of sacrifice was determined for most groups of mice (number positive/number tested). When sera were assayed as pools, the presence or absence of P39 IgG was scored as + or −, respectively. ND, not determined.
c
Data were not recorded for one mouse in groups B3 and E3 that died prior to challenge.
d
Data for experiments C and E are reproduced from Vaccines ’97 (10a) with permission of the publisher.