TABLE 2.
Expt (mouse strain/challenge isolate) | Mouse group | Immunogena | No. of positive cultures/total no. tested
|
P39 IgGb | No. of mice infected/total no. | ||
---|---|---|---|---|---|---|---|
Bladder | Ear | Joint | |||||
A (C3H/297) | 1 | His-DbpA297 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 |
2 | Lpp1:DbpA297 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | |
3 | OspAB31 | 0/5 | 0/5 | 0/5 | − | 0/5 | |
4 | PBS | 5/5 | 5/5 | 4/5 | + | 5/5 | |
B (C3H/N40) | 1 | Lpp2:DbpAN40His | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 |
2 | OspAB31 | 5/5 | 0/5 | 0/5 | + | 5/5 | |
3 | E. coli BL21 (det.) | 4/4 | 4/4 | 1/4 | + | 4/4c | |
4 | None | 5/5 | 5/5 | 5/5 | + | 5/5 | |
Cd (BALB/B31) | 1 | Pre-DbpA297 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 |
2 | OspAB31 | 0/5 | 0/5 | 0/5 | − | 0/5 | |
3 | E. coli JM101 (sol.) | 5/5 | 5/5 | 5/5 | ND | 5/5 | |
4 | None | 5/5 | 5/5 | 5/5 | + | 5/5 | |
(C3H/B31) | 5 | Pre-DbpA297 | 5/5 | 0/5 | 0/5 | 5/5 | 5/5 |
6 | OspAB31 | 0/5 | 0/5 | 0/5 | − | 0/5 | |
7 | E. coli JM101 (sol.) | 5/5 | 5/5 | 5/5 | ND | 5/5 | |
8 | None | 5/5 | 5/5 | 5/5 | + | 5/5 | |
D (C3H/B31) | 1 | Pre-DbpA297 | 5/5 | 0/5 | 0/5 | 5/5 | 5/5 |
2 | Lpp1:DbpA297 | 5/5 | 0/5 | 0/5 | 5/5 | 5/5 | |
3 | OspAB31 | 0/5 | 0/5 | 0/5 | − | 0/5 | |
4 | E. coli JM101 (sol.) | 5/5 | 5/5 | 5/5 | + | 5/5 | |
5 | E. coli BL21 (det.) | 5/5 | 5/5 | 5/5 | + | 5/5 | |
6 | None | 5/5 | 5/5 | 5/5 | + | 5/5 | |
Ed (C3H/B31) | 1 | Pre-DbpA297 | 3/5 | 0/5 | 0/5 | 3/5 | 3/5 |
2 | His-DbpA297 | 1/5 | 0/5 | 1/5 | 1/5 | 1/5 | |
3 | His-DbpAB31 | 1/4 | 0/4 | 0/4 | 0/4 | 1/4c | |
4 | OspAB31 | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | |
5 | E. coli M15 (sol.) | 5/5 | 5/5 | 5/5 | ND | 5/5 | |
6 | None | 5/5 | 5/5 | 5/5 | 5/5 | 5/5 |
Immunogen doses were 20 μg for DbpA, 5 μg for OspA, and 5 μg for E. coli soluble (sol.) and detergent (det.) extracts, except 20 μg in experiment C.
Presence of IgG to P39 at the time of sacrifice was determined for most groups of mice (number positive/number tested). When sera were assayed as pools, the presence or absence of P39 IgG was scored as + or −, respectively. ND, not determined.
Data were not recorded for one mouse in groups B3 and E3 that died prior to challenge.
Data for experiments C and E are reproduced from Vaccines ’97 (10a) with permission of the publisher.