Figure 6. Live/dead staining of ADSCs on the alginate and collagen stacks. (A–F) Inverted epifluorescent microscope images after live/dead staining of ADSCs on the alginate stacks. (A) Slice 1 (top slice) showing some good cell viability on day 1. (B) Slice 2 (middle slice) showing very limited cell presence indicating no cell mobility through the construct on day 1. (C) Slice 3 (bottom slice) showing no cells and thus no mobility through the cross-linked alginate on day 3. (D) Slice 1 (top slice) showing good cell viability with some stain intake by the alginate on day 7. (E) Slice 2 (middle slice) showing limited cell viability and thus mobility on day 7. (F) Slice 3 (bottom slice) showing no cell viability and thus no mobility through the alginate even on day 7. (G–L) inverted epifluorescent microscope images after live/dead staining of ADSCs on the collagen stacks. (G) Slice 1 (top slice) showing good cell viability on day 1. (H) Slice 2 (middle slice) showing good cell viability and therefore cells must be able to travel through on day 1. (I) Slice 3 (bottom slice) showing good cell viability and thus demonstrating the mobility of cells through the collagen and the micromachined fibre mesh even after 1 day. (J) Slice 1 (top slice) showing good cell viability on day 4. (K) Slice 2 (middle slice) showing good cell viability and therefore cells must be able to travel through on day 4. (L) Slice 3 (bottom slice) showing good cell viability and thus demonstrating the mobility of cells through the collagen and the micromachined fibre mesh on day 4. Scale bars: 500 μm. ADSC: adipose derived stem cell.
