FIG. 5.

Immunoprecipitation of ETA with peptide-specific antibodies. ETA preincubated with serum from group IV (immunized with ETA) rabbits (lane 1), group I (binding domain) rabbits (lane 2), group II (translocation domain) rabbits (lane 3), group IIIa (enzymatic domain) rabbits (lane 4), group IIIb (enzymatic) rabbits (lane 5), preimmune rabbit serum (lane 6), and PBS (lane 7) was precipitated with recombinant protein G, separated on a 12% polyacrylamide gel, transferred to a nitrocellulose membrane, and stained with anti-ETA antibodies (0.25 μg/ml). Lane 8 represents pure ETA (2 μg) directly loaded onto the gel.